KMID : 1023420100100050365
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Journal of Dental Hygiene Science 2010 Volume.10 No. 5 p.365 ~ p.372
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Antioxidative Activities and Antiproliferation Effects on Oral Carcinoma KB Cell of the Brazilin from Caesalpinia sappan L.
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Jeon Mi-Ae
Kwon Hyun-Jung Kim Young-Hyun Han Kook-Il Chung Ha-Na Chung Sung-Kyun Bang In-Soek Han Man-Deuk
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Abstract
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Caesalpinia sappan L. has long been commonly used in oriental folk medicines and as dyes materials. To investigate the antioxidative activities and antiproliferation effects of brazilin from C. sappan heart wood, the MeOH soluble extract was successively fractionated by using hexane, CHCl3, EtOAc, BuOH, MeOH, and H2O. In these fractions, we were purified brazilin from EtOAc fraction which partitioned to 3.94% of the highest yields. The effects of brazilin and the extracts on human oral carcinoma cells (KB) by MTT assay and their antioxidant activities by DPPH, TCA assay and Fenton reaction were tested. The results showed that the brazilin could inhibits the proliferation of KB cells and obviously decreased the production of nitric oxide of the cells. When the concentration of the brazilin reached to 100 ¥ìg/ml, the inhibition percentage of the cell growth was about 60%. In assay on antioxidant activities, The results showed that brazilin exhibit the highest capacity of DPPH free radical scavenging effects among tested extracts. When the concentration of brazilin reached to 1 mg/ml, the lipid peroxide inhibition and radical inhibition activities were determined to be 65.0% and 85.8%, respectively. These results are suggest that brazilin have stronger antiproliferation effect on KB cell and antioxidant properties.
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KEYWORD
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Antioxidative activities, Antiproliferation effect, Caesalpinia sappan, Oral carcinoma KB cell
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